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vitro dsrna synthesis  (New England Biolabs)


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    Structured Review

    New England Biolabs vitro dsrna synthesis
    Vitro Dsrna Synthesis, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsrna+synthesis/T7+RNA+Polymerase/pm41628996-115-19-26
    Average 99 stars, based on 3521 article reviews
    vitro dsrna synthesis - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: IPS (In-Plant System) Delivery of Double-Stranded Vitellogenin and Vitellogenin receptor via Hydroponics for Pest Control in Diaphorina citri Kuwayama (Hemiptera: Psyllidae).
    Article Snippet: The purified PCR Vg4 fragments were inserted into the plasmid of the pMD18T vector (DH5α, Thermo Fisher Scientific, Waltham, Massachusetts, USA). .. The resulting Vg4 plasmids were used as templates to generate dsVg4 with the dsRNA synthesis kit (RNAsyn Biottech Co., Ltd., Jiangsu, Suzhou, China; Supplementary Materials), following the manufacturer’s instructions: the preparation of dsRNA synthesis (DEPC water 51.6 μL, 5× transcription buffer 24 μL, NTP 20.4 μL, transcriptional template 6 μL, RNase enzyme inhibitor 3.6 μL, DTT 2.4 μL, RNA polymerase 12 μL), transcript at 37 ◦C for 1 h, add 12 μL DNaseI and react at 37 ◦C for 30 min, remove the template, and purify using NEB’s RNA purification kit (NEB, Ipswich, Massachusetts, USA) to obtain positive and negative Vg4 RNA; mix positive and negative Vg4 RNA in equal proportions and anneal to obtain dsVg4, with detailed synthesis methods provided in the Supplementary Materials. .. Synthesized dsRNA was quantitated by an N80 Touch nanophotometer (Implen, Munich, Bavaria, Germany) at 260 nm, and the integrity was analyzed by agarose gel electrophoresis.

    Article Title: IPS (In-Plant System) Delivery of Double-Stranded Vitellogenin and Vitellogenin receptor via Hydroponics for Pest Control in Diaphorina citri Kuwayama (Hemiptera: Psyllidae)
    Article Snippet: The purified PCR Vg4 fragments were inserted into the plasmid of the pMD18T vector (DH5α, Thermo Fisher Scientific, Waltham, Massachusetts, USA). .. The resulting Vg4 plasmids were used as templates to generate ds Vg4 with the dsRNA synthesis kit (RNAsyn Biottech Co., Ltd., Jiangsu, Suzhou, China; ), following the manufacturer’s instructions: the preparation of dsRNA synthesis (DEPC water 51.6 μL, 5× transcription buffer 24 μL, NTP 20.4 μL, transcriptional template 6 μL, RNase enzyme inhibitor 3.6 μL, DTT 2.4 μL, RNA polymerase 12 μL), transcript at 37 °C for 1 h, add 12 μL DNaseI and react at 37 °C for 30 min, remove the template, and purify using NEB’s RNA purification kit (NEB, Ipswich, Massachusetts, USA) to obtain positive and negative Vg4 RNA; mix positive and negative Vg4 RNA in equal proportions and anneal to obtain ds Vg4 , with detailed synthesis methods provided in the . .. Synthesized dsRNA was quantitated by an N80 Touch nanophotometer (Implen, Munich, Bavaria, Germany) at 260 nm, and the integrity was analyzed by agarose gel electrophoresis.

    Polymerase Chain Reaction:

    Article Title: Wun2-mediated integrin recycling promotes apoptotic cell clearance in Drosophila melanogaster.
    Article Snippet: Apoptotic cell (AC) clearance is a complex process in which phagocytes recognize, engulf, and digest ACs during organismal development and tissue homeostasis.. Impaired efferocytosis results in developmental defects and autoimmune diseases.. In the current study, we performed RNA-sequencing to systematically identify regulators involved in the phagocytosis of ACs by Drosophila melanogaster macrophage-like S2 cells, followed by targeted RNA interference screening.

    Generated:

    Article Title: Wun2-mediated integrin recycling promotes apoptotic cell clearance in Drosophila melanogaster.
    Article Snippet: Apoptotic cell (AC) clearance is a complex process in which phagocytes recognize, engulf, and digest ACs during organismal development and tissue homeostasis.. Impaired efferocytosis results in developmental defects and autoimmune diseases.. In the current study, we performed RNA-sequencing to systematically identify regulators involved in the phagocytosis of ACs by Drosophila melanogaster macrophage-like S2 cells, followed by targeted RNA interference screening.

    Amplification:

    Article Title: Wun2-mediated integrin recycling promotes apoptotic cell clearance in Drosophila melanogaster.
    Article Snippet: Apoptotic cell (AC) clearance is a complex process in which phagocytes recognize, engulf, and digest ACs during organismal development and tissue homeostasis.. Impaired efferocytosis results in developmental defects and autoimmune diseases.. In the current study, we performed RNA-sequencing to systematically identify regulators involved in the phagocytosis of ACs by Drosophila melanogaster macrophage-like S2 cells, followed by targeted RNA interference screening.



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